KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay kit
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CAT.: A1090012D
KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between BRD4 protein and the VHL/Elongin C/Elongin B protein complex (VHL/Elongin C/Elongin B protein complex, hereinafter referred to as the VCB complex). This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑VCB Complex protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑BRD4 protein. The Tag1‑VCB Complex protein and the Tag2‑BRD4 protein can form a ternary complex mediated by the positive reference PROTAC molecule MZ‑1, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BRD4 and the VCB Complex.
| CAT. | Description | Size |
| A1090012D | KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay kit | 100 tests |
|
• 1 vial （5 μL/vial） VHL binding standard 1 • 1 vial （8 μL/vial） Tag1-VCB Complex • 1 vial （8 μL/vial） Tag2-BRD4 • 1 vial （5 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （5 μL/vial） mAb anti-Tag2 - LA • 1 vial （5 μL/vial） TCEP (1 M) • 2 vials （2 mL/vial） PROTAC Diluent Buffer #2 • 1 vial （2 mL/vial） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between BRD4 protein and the VHL/Elongin C/Elongin B protein complex (VHL/Elongin C/Elongin B protein complex, hereinafter referred to as the VCB complex). This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑VCB Complex protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑BRD4 protein. The Tag1‑VCB Complex protein and the Tag2‑BRD4 protein can form a ternary complex mediated by the positive reference PROTAC molecule MZ‑1, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BRD4 and the VCB Complex.
| CAT. | Description | Size |
| A1090012S | KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay kit | 500 tests |
|
• 1 vial （10 μL/vial） VHL binding standard 1 • 1 vial （40 μL/vial） Tag1-VCB Complex • 1 vial （40 μL/vial） Tag2-BRD4 • 1 vial （25 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （25 μL/vial） mAb anti-Tag2 - LA • 1 vial （120 μL/vial） TCEP (1 M) • 1 bottle （50 mL/bottle） PROTAC Diluent Buffer #2 • 1 bottle （30 mL/bottle） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between BRD4 protein and the VHL/Elongin C/Elongin B protein complex (VHL/Elongin C/Elongin B protein complex, hereinafter referred to as the VCB complex). This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑VCB Complex protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑BRD4 protein. The Tag1‑VCB Complex protein and the Tag2‑BRD4 protein can form a ternary complex mediated by the positive reference PROTAC molecule MZ‑1, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BRD4 and the VCB Complex.
| CAT. | Description | Size |
| A1090012L | KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay kit | 5,000 tests |
|
• 1 vial （50 μL/vial） VHL binding standard 1 • 1 vial （400 μL/vial） Tag1-VCB Complex • 1 vial （400 μL/vial） Tag2-BRD4 • 1 vial （250 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （250 μL/vial） mAb anti-Tag2 - LA • 2 vials （120 μL/vial） TCEP (1 M) • 1 bottle （200 mL/bottle） PROTAC Diluent Buffer #2 • 1 bottle （120 mL/bottle） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
English
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